Development and Validation of a Quantitative PCR for Detection of California Sea Lion Adenovirus 1
- Infectious disease
- Diagnostics
Abstract
California sea lion adenovirus 1 (CSLAdV-1) has been associated with hepatitis and enteritis in several wild and captive populations of diverse pinniped species. Currently available tests have been limited to pan-adenoviral polymerase chain reaction (PCR) followed by sequencing. We present the development of a quantitative probe-hybridization PCR (qPCR) assay for rapid, sensitive, and specific detection of this virus in California sea lions (Zalophus californianus) and other pinnipeds. This assay did not amplify other mammalian adenoviruses and is able to detect consistently down to 10 viral copies per well. Compared with the gold standard conventional pan-adenovirus PCR/sequencing assay, diagnostic sensitivity and specificity of 100% and 88.2% were found, respectively. The lower diagnostic specificity of this qPCR assay may be the result of the lower limit of detection of this assay compared with the gold standard rather than the result of detection of true false-positives.
Cortes-Hinojosa, G., Gulland, F.M.D., Goldstein, T., Venn-Watson, S., Rivera, R., Archer, L.L., Waltzek, T.B., Gray, G.C., Wellehan Jr., J.F.X. 2017. Development and validation of a quantitative PCR for rapid and specific detection of California sea lion adenovirus 1 and prevalence in wild and managed populations. Journal of Veterinary Diagnostic Investigation. 29(2): 193-197
diagnostics
Related Publications
{"image":"\/Animals\/Patients\/California sea lions\/csl-by-bill-hunnewell-c-the-marine-mammal-center-1.jpg","alt":"California sea lion eating a fish","title":"New Technique for Diagnosing Lung Parasite Infections in Sea Lions","link_url":"https:\/\/www.marinemammalcenter.org\/publications\/new-technique-for-diagnosing-lung-parasite-infections-in-sea-lions","label":"Research Paper"}
{"image":"\/Animals\/Patients\/Elephant seals\/cropped-images\/es-by-bill-hunnewell-c-the-marine-mammal-center-3-2-1214-3454-3512-1600891002.jpg","alt":"northern elephant seal","title":"Diagnostic Tests for Lungworm-Infected Northern Elephant Seals","link_url":"https:\/\/www.marinemammalcenter.org\/publications\/diagnostic-tests-for-lungworm-infected-northern-elephant-seals","label":"Research Paper"}
{"image":"\/People\/Action\/Veterinary care\/cropped-images\/cara-field-fur-seal-exam-by-bill-hunnewell-c-the-marine-mammal-center-0-0-1245-1000-1601838274.jpg","alt":"Dr. Cara Field takes blood from a fur seal","title":"Evaluating Diagnostic Tools for White Blood Cell Counts in Pinnipeds","link_url":"https:\/\/www.marinemammalcenter.org\/publications\/evaluating-diagnostic-tools-for-white-blood-cell-counts-in-pinnipeds","label":"Research Paper"}
{"image":"\/Animals\/Wild\/Other species\/cropped-images\/fin-whale-fort-funston-by-barbie-halaska-c-the-marine-mammal-center-259-328-3782-2954-1619455378.jpg","alt":"","title":"Analyzing Stress and Reproductive Steroid Hormones in Gray and Fin Whales","link_url":"https:\/\/www.marinemammalcenter.org\/publications\/analyzing-stress-and-reproductive-steroid-hormones-in-gray-and-fin-whales","label":"Research Paper"}
Recent News
{"image":"\/Animals\/Patients\/Harbor seals\/2013\/cropped-images\/hs-bogey-by-ingrid-overgard-c-the-marine-mammal-center-371-0-2712-2118-1607385758.jpg","alt":"harbor seal Bogey","title":"Top Holiday Gifts for Ocean Lovers","link_url":"https:\/\/www.marinemammalcenter.org\/news\/top-eco-friendly-holiday-gifts-that-give-back","label":"News Update","date":"2025-11-04 01:00:00"}
{"image":"\/Animals\/Wild\/Hawaiian monk seal\/cropped-images\/hms-wild-photo-1-c-noaa-pifsc-hmsrp-35-0-1270-992-1759760452.jpg","alt":"A Hawaiian monk seal rests on its side on a sandy beach.","title":"Where Do Hawaiian Monk Seals Live? And Other \u2018\u012alio Holo I Ka Uaua Trivia","link_url":"https:\/\/www.marinemammalcenter.org\/news\/where-do-hawaiian-monk-seals-live-and-other-ilio-holo-i-ka-uaua-trivia","label":"News Update","date":"2025-10-06 00:00:00"}
Where Do Hawaiian Monk Seals Live? And Other ‘Īlio Holo I Ka Uaua Trivia
October 6, 2025
Read More{"image":"\/Animals\/Patients\/California sea lions\/2025\/cropped-images\/dottie-csl-photo-by-bill-hunnewell-218-0-1270-992-1759777087.jpg","alt":"California sea lion","title":"LA Times: Marine mammals are dying in record numbers along the California coast","link_url":"https:\/\/www.marinemammalcenter.org\/news\/la-times-marine-mammals-are-dying-in-record-numbers-along-the-california-coast","label":"In the News","date":"2025-10-03 02:00:00"}
LA Times: Marine mammals are dying in record numbers along the California coast
October 3, 2025
Read More{"image":"\/People\/Action\/Veterinary care\/cropped-images\/dane-whitaker-and-emily-whitmer-vaccine-trial-by-bill-hunnewell-49-0-1270-992-1757364612.jpg","alt":"Two veterinarians collect a blood sample from an elephant seal in a rehabilitation pen.","title":"Bird Flu Vaccine Trial May Offer Hope for Protecting Hawaiian Monk Seals","link_url":"https:\/\/www.marinemammalcenter.org\/news\/bird-flu-vaccine-trial-may-offer-hope-for-protecting-hawaiian-monk-seals","label":"News Update","date":"2025-09-09 09:13:00"}
Bird Flu Vaccine Trial May Offer Hope for Protecting Hawaiian Monk Seals
September 9, 2025
Read More