Skip to main content

Your Impact Will Go Twice as Far for a Pup

Do you notice all those folds of skin? They tell a heartbreaking story of a pup far too thin. 

Right now, there’s a $5,000 gift match waiting for the pups, and your support is needed to unlock it. 

Yes, your $5 = $10 today, meaning you can provide double the fish meals for a pup like Horseshoe. 

Get your gift matched
elephant seal pup Horseshoe
California sea lion mom and pup

Effects of In Vitro Exposure to PCBs on California Sea Lions

Effects of polychlorinated biphenyls (PCB) on California sea lion (Zalophus californianus) lymphocyte functions upon in vitro exposure
  • Cancer


Polychorinated biphenyl (PCB) congeners are a cause for concern due to their persistence in the environment, their lipophilic properties that cause them to bio-accumulate in top predators, and their adverse effects on mammalian health. For example, the common urogenital carcinoma reported in California sea lions (Zalophus californianus) (CSL) is associated with high tissue levels of PCBs, but the mechanisms responsible for this association are unknown. This study investigated the effect of exposure to six PCB congeners and a congener mix at low and environmentally relevant concentrations on NK cell-like and T cell activity using in vitro assays on cryopreserved lymph node mononuclear cells isolated from dead CSL. Non dioxin-like congeners 153 and 180 increased lymphocyte proliferation at 5 and 10 ppm, while congener 138 decreased proliferation by up to 43% at 15 ppm. Dioxin-like PCBs 118 and 169 did not affect lymphocyte proliferation, while the effects of congener 105 depended on the mitogen concentration; these did not correlate with their predicted toxic equivalent factors. NK cell-like activity was affected only by the highest concentration of PCBs tested; it was increased by non-dioxin-like congeners 138 and 153, and decreased by dioxin-like congener 169. The PCB congener mix suggested that the effects of PCB congeners were not simply additive. Our results concur with effects of PCBs reported for other pinniped's lymphocytes and add further experimental support to the observation that dioxin-like PCBs are not the most toxic congeners for marine mammals, contrary to effects in other species. This is the first evidence of in vitro suppression of NK cell-like cytotoxicity by a dioxin-like congener in a pinniped. More importantly, the observed results suggest that PCBs can modulate the CSL immune system, increasing exposed individuals' susceptibility to viral and oncogenic challenges. 

Peñín, I., Levin, M., Acevedo-Whitehouse, K., Jasperse, L., Gebhard, Gulland, F.M.D.,and S. De Guise. 2018. Effects of polychlorinated biphenyls (PCB) on California sea lion (Zalophus californianus,) lymphocyte functions upon in vitro exposure. Environmental Research 167:708-717.


Related Publications

Related News