Genetics and Pathology Associated with Klebsiella Pneumonia
- Infectious disease
- Pathology
Abstract
Southern sea otters (SSO: Enhydra lutris nereis) are a federally-listed threatened subspecies found almost exclusively in California, USA. Despite their zoonotic potential and lack of host specificity, K. pneumoniae and Klebsiella spp. have largely unknown epizootiology in SSOs. Klebsiella pneumoniae is occasionally isolated at necropsy, but not from live SSOs. Hypermucoviscous (HMV) K. pneumoniae strains are confirmed pathogens of Pacific Basin pinnipeds, but have not been previously isolated from SSOs. We characterized the virulence profiles of K. pneumoniae isolates from necropsied SSOs, evaluated killing of marine mammal K. pneumoniae following in vitro exposure to California sea lion (CSL: Zalophanus californianus) whole blood and serum, and characterized lesion patterns associated with Klebsiella spp. infection in SSOs. Four of 15 SSO K. pneumoniae isolates were HMV and all were recovered from SSOs that stranded during 2005. Many K. pneumoniae infections were associated with moderate to severe pathology as a cause of death or sequela. All HMV infections were assessed as a primary cause of death or as a direct result of the primary cause of death. Klebsiella-infected SSOs exhibited bronchopneumonia, tracheobronchitis and/or pleuritis, enteritis, Profilicollis sp. acanthocephalan peritonitis, septic peritonitis, and septicemia. All SSO HMV isolates were capsular type K2, the serotype most associated with HMV infections in CSLs. Multiplex PCR revealed two distinct virulence gene profiles within HMV isolates and two within non-HMV isolates. In vitro experiments investigating CSL whole blood and serum killing of K. pneumoniae suggest that HMV isolates are more resistant to serum killing than non-HMV isolates.
Chang, E.K., Miller, M., Shahin, K., Batac, F., Field, C.L., Duignan, P., Struve, C., Byrne, B.A., Murray, M.J., Greenwald, K. and Smith, W.A., 2021. Genetics and pathology associated with Klebsiella pneumoniae and Klebsiella spp. isolates from North American Pacific coastal marine mammals. Veterinary Microbiology, p.109307.
pathology
Cara Field
Pádraig Duignan
Meet The Experts
{"image":"\/People\/Portrait\/cara-field.jpg","alt":"Cara Field","title":"Cara Field","text":"Medical Director","link_url":"https:\/\/www.marinemammalcenter.org\/person\/cara-field","link_text":"Read Bio"}
{"image":"\/People\/Portrait\/cropped-images\/Padraig Duignan-0-56-635-496-1601760621.jpg","alt":"Padraig Duignan","title":"P\u00e1draig Duignan","text":"Director of Pathology","link_url":"https:\/\/www.marinemammalcenter.org\/person\/padraig-duignan","link_text":"Read Bio"}
Related Publications
{"image":"\/Animals\/Patients\/Elephant seals\/es-photo-by-bill-hunnewell-c-the-marine-mammal-center-1.jpg","alt":"northern elephant seal","title":"Muscle-Wasting Disease in Elephant Seal Pups","link_url":"https:\/\/www.marinemammalcenter.org\/publications\/muscle-wasting-disease-in-elephant-seal-pups","label":"Research Paper"}
{"image":"\/Animals\/Wild\/Bottlenose dolphin\/bottlenose-dolphins-leaping-shutterstock.jpg","alt":"dolphins leaping out of the water","title":"Fresh Water Skin Disease in Dolphins","link_url":"https:\/\/www.marinemammalcenter.org\/publications\/fresh-water-skin-disease-in-dolphins","label":"Research Paper"}
{"image":"\/Animals\/Wild\/Sea otter\/cropped-images\/sea-otter-grooming-head-shutterstock-22-86-3617-2826-1616709449.jpg","alt":"sea otter grooming","title":"Parasitic Skin Disease in Threatened Southern Sea Otters","link_url":"https:\/\/www.marinemammalcenter.org\/publications\/parasitic-skin-disease-in-threatened-southern-sea-otters","label":"Research Paper"}
{"image":"\/Animals\/Wild\/Other species\/cropped-images\/galapagos-sea-lion-shutterstock-954-376-2988-2334-1617750524.jpg","alt":"Galapagos sea lion","title":"Mycoplasmas in Galapagos Sea Lions and Their Association with Other Respiratory Pathogens","link_url":"https:\/\/www.marinemammalcenter.org\/publications\/occurrence-of-mycoplasmas-in-galapagos-sea-lions","label":"Research Paper"}
Mycoplasmas in Galapagos Sea Lions and Their Association with Other Respiratory Pathogens
Read MoreRelated News
{"image":"\/Animals\/Wild\/Humpback whale\/cropped-images\/whale-tail-with-bridge-and-ships-shutterstock-3-7-4898-3826-1663722256.jpg","alt":"whale tails visible above water under the Golden Gate Bridge with shipping traffic in the distance","title":"Whale Safe \u2013 an AI-enabled System to Prevent Ship Collisions with Whales \u2013 to Launch off the San Francisco Coastline","link_url":"https:\/\/www.marinemammalcenter.org\/news\/whale-safe-to-launch-off-the-san-francisco-coastline","label":"Press Release","date":"2022-09-21 04:00:00"}
Whale Safe – an AI-enabled System to Prevent Ship Collisions with Whales – to Launch off the San Francisco Coastline
September 21, 2022
Read More{"image":"\/Animals\/Wild\/Humpback whale\/cropped-images\/two-whales-under-golden-gate-bridge-shutterstock-646-1-3946-3083-1663782644.jpg","alt":"two whales surfacing below the Golden Gate Bridge","title":"New York Times: New System Aims to Save Whales Near San Francisco From Ship Collisions","link_url":"https:\/\/www.marinemammalcenter.org\/news\/new-york-times-new-system-aims-to-save-whales-near-san-francisco-from-ship-collisions","label":"In the News","date":"2022-09-21 02:00:00"}
New York Times: New System Aims to Save Whales Near San Francisco From Ship Collisions
September 21, 2022
Read More{"image":"\/Animals\/Wild\/Humpback whale\/cropped-images\/fran-humpback-whale-photo-c-doug-croft-blue-ocean-whale-watch-473-0-2161-1688-1661961931.jpg","alt":"Fran the humpback whale leaping out of the water","title":"Humpback Whale Killed by Suspected Ship Strike Was One of the Most Well-Known Whales in California","link_url":"https:\/\/www.marinemammalcenter.org\/news\/humpback-whale-killed-by-suspected-ship-strike-was-one-of-the-most-well-known-whales-in-california","label":"Press Release","date":"2022-08-31 02:00:00"}
Humpback Whale Killed by Suspected Ship Strike Was One of the Most Well-Known Whales in California
August 31, 2022
Read More{"image":"\/Animals\/Wild\/Humpback whale\/cropped-images\/humpback-whale-breach-photo-c-bill-hunnewell-422-0-3324-2597-1658185516.jpg","alt":"humpback whale breaching","title":"Forbes: Huge Humpback Whale\u2014And California Celebrity\u2014Killed In Ship Strike Amid Concern Over Collisions","link_url":"https:\/\/www.marinemammalcenter.org\/news\/forbes-huge-humpback-whaleand-california-celebritykilled-in-ship-strike-amid-concern-over-collisions","label":"In the News","date":"2022-08-31 02:00:00"}
Forbes: Huge Humpback Whale—And California Celebrity—Killed In Ship Strike Amid Concern Over Collisions
August 31, 2022
Read More